Assessing Helicobacter pylori motility and biofilm formation in subinhibitory concentrations of antimicrobials

Numerous studies have shown that subinhibitory concentrations of antimicrobials can alter bacterial virulence factors. This study evaluates motility and biofilm formation by H. pylori 43504 grown in amoxicillin (AMX), clarithromycin (CLA), or tetracycline (TET). For the swimming swarming assays, suspensions were prepared with strain alone AMX, CLA, TET at ½ MIC. Next, media incubated 37 ºC, under microaerophilia. To assess presence one antimicrobial concentrations, (109 CFU/mL) 2.5% FBS containing After incubation for 10 days, medium MIC presented greater lower than non-treated strain. showed stronger production Our results favors after 3 days. may clinical consequences make microorganism difficult to eradicate.